New tests for characterization of mumps virus antibodies: hemolysis inhibition, single radial immunodiffusion with immobilized virions, and mixed hemadsorption.
نویسندگان
چکیده
Hemolysis inhibition (HLI), single radial immunodiffusion (SRID) with immobilized virions, and mixed hemadsorption tests were used for measuring antibodies against mumps virus. Rabbit hyperimmune sera against mumps and early and late human convalescent sera were analyzed. All three tests identified antibodies against both hemagglutinin and the second major envelope component, hemolysin (fusion factor). The sensitivity of the HLI test corresponded to that of the hemagglutination inhibition (HI) test, but in some sera HLI antibodies occurred in greater quantity than HI antibodies. The SRID test readily identified rises in antibody titers in connection with acute infection. Due to its simplicity and lack of sensitivity to nonspecific inhibitors, it is recommended for use in this context. The mixed hemadsorption test showed a high sensitivity for specific identification of mumps antibodies. It therefore may be suitable for use in screening for immunity to mumps.
منابع مشابه
Comparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies
متن کامل
Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus.
A hemolysis-in-gel test for the demonstration of antibodies to mumps virus is described. The results were compared with those of neutralization tests using a modified microtechnique. In the neutralization test viral replication was demonstrated by the hemadsorption of guinea pig erythrocytes, the visibility of which could be further enhanced by the use of o-tolidine. Good correlation was found ...
متن کاملUdk 619:616.988.73:636.5 Examination of the Activity of Hn and F Glycoprotein Antigens of the Outer Envelope of Newcastle Disease Virus by Using Fusional, Hemolytic, Hemagglutination and Hemadsorption Tests, in Vitro
The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND vir...
متن کاملComparison of antibodies against different viruses in cerebrospinal fluid and serum samples from patients with multiple sclerosis.
The occurrence of a local production in the central nervous system (CNS) of antibodies against different selected viruses was analyzed by comparison of titers in serum and cerebrospinal fluid samples from groups of 50 patients with multiple sclerosis from Finland, Norway, and Sweden. Measles antibodies were determined in hemagglutination inhibition, hemolysis inhibition, and nucleocapsid comple...
متن کاملComparative measurement of equine influenza virus antibodies in horse sera by single radial hemolysis, neutralization, and hemagglutination inhibition tests.
Single radial hemolysis (SRH), neutralization (NT), and hemagglutination inhibition (HI) tests were carried out on sera from horses immunized against the Prague and Miami strains of equine influenza virus. The HI and NT tests demonstrated good sensitivity; the sensitivity of the SRH test was somewhat lower. The NT titers of individual sera were correlated very closely with the HI titers, althou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 5 3 شماره
صفحات -
تاریخ انتشار 1977